Search Published Scientific Projects Competitive Products and Technology Patents
Login
Title:
RECOMBINANT MICROORGANISMS FOR ENHANCED PRODUCTION OF MEVALONATE, ISOPRENE, AND ISOPRENOIDS
Number:
WO2012149491A2
Publication Date:
2012-11-01
Location:
USA
Priority Date:
IPC clasifications:
C12N 1/21; C12N 9/00; C12N 9/10; C12N 9/12; C12N 9/18; C12N 9/88; C12P 5/00; C12P 7/02; C12P 7/04; C12P 7/42
Assignee:
DANISCO US INC.|THE GOODYEAR TIRE & RUBBER COMPANY
Inventor:
BECK, Zachary, Q.|MILLER, Michael, C.|PERES, Caroline, M.|PRIMAK, Yuliya, A.|PUCCI, Jeff, P.|WELLS, Derek, H.
Class / subclass:
Integration and development / Other useful products obtained
Industry:
Chemical
Abstract:

The invention features compositions and methods for the increased production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids in microorganisms by engineering a microorganism for increased carbon flux towards mevalonate production in the following enzymatic pathways: (a) citrate synthase, (b) phosphotransacetylase, © acetate kinase, (d) lactate dehydrogenase, (e) malic enzyme, and (f) pyruvate dehydrogenase such that one of more of the enzyme activity is modulated. In addition, production of mevalonate, isoprene, isoprenoid precursor molecules, and/or isoprenoids can be further enhanced by the heterologous expression of the mvaE and mvaS genes (such as, but not limited to, mvaE and mvaS genes from the organisms Listeria grayi DSM 20601, Enterococcus faecium, Enterococcus gallinarum EG2, and Enterococcus casseliflavus).

Claims:

NA

Patent Family:
US20120276587A1 WO2012149491A2 WO2012149491A3