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Title:
SYNTHETIC BIOLOGY TOOLS
Number:
US20130005590A1
Publication Date:
2013-01-03
Location:
USA
Priority Date:
IPC clasifications:
C12N 1/19; C12N 1/21; C12N 5/10; C40B 30/02; C40B 50/00; C12N 15/00
Assignee:
The Regents of the University of California
Inventor:
Lou; Chunbo|Moon; Tae Seok|Rhodius; Virgil|Stanton; Brynne|Tamsir; Alvin|Temme; Karsten|Voigt; Chirs
Class / subclass:
Enabling Technologies
Industry:
Biomedicine
Abstract:

Methods for design of genetic circuits are provided.

Claims:

A method of designing a genetic circuit containing one or more orthogonal sequence-specific DNA binding polypeptides, the method comprising providing a set of sequence-specific DNA binding polypeptides; optimising expression of the polypeptides in a heterologous host cell; identifying target DNA sequences to which the polypeptides bind; generating synthetic transcriptional regulatory elements comprising at least one identified target DNA sequence, wherein the regulatory elements are responsive to a sequence-specific DNA binding polypeptide from the set of sequence-specific DNA binding polypeptides; designing cognate sequence-specific DNA binding polypeptide-target DNA sequence pairs to generate a set of orthogonal sequence-specific DNA binding polypeptide-target DNA sequence pairs; designing a genetic circuit containing one or more orthogonal sequence-specific DNA binding polypeptide-target DNA sequence pairs from the set of orthogonal sequence-specific DNA binding polypeptide-target DNA sequence pairs, thereby designing a genetic circuit containing one or more orthogonal sequence-specific DNA binding polypeptides.

Patent Family:
US20130005590A1 WO2012170436A1