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Title:
Production of odd chain fatty acid derivatives in recombinant microbial cells
Number:
US8372610
Publication Date:
2013-02-12
Location:
USA
Priority Date:
IPC clasifications:
C12N 1/20; C12N 15/74; C12P 7/64
Assignee:
LS9, Inc.
Inventor:
LEE, Grace J.|HALIBURTON, John R.|HU, Zhihao|SCHIRMER, Andreas W.
Class / subclass:
Integration and development / Precompetitive stage
Industry:
Life Sciences
Abstract:

Recombinant microbial cells are provided which have been engineered to produce fatty acid derivatives having linear chains containing an odd number of carbon atoms by the fatty acid biosynthetic pathway. Also provided are methods of making odd chain fatty acid derivatives using the recombinant microbial cells, and compositions comprising odd chain fatty acid derivatives produced by such methods.

Claims:

1. A recombinant E. coli host cell transformed with:

(a) polynucleotides encoding polypeptides having enzymatic activity effective to produce an increased amount of propionyl-CoA in the recombinant E. coli host cell compared to the amount of propionyl-CoA produced in a parental E. coli host cell lacking or having a reduced amount of said enzymatic activity, wherein said polynucleotides encode polypeptides having aspartokinase activity, homoserine dehydrogenase activity, homoserine kinase activity, threonine synthase activity, and threonine deaminase activity,

(b) a polynucleotide encoding a polypeptide having β-ketoacyl-ACP synthase activity that utilizes propionyl-CoA as a substrate and has 80% identity to SEQ ID NO:7, and

© a polynucleotide encoding a polypeptide having thioesterase enzyme activity,

wherein the recombinant E. coli host cell produces a fatty acid composition comprising odd-number chain length fatty acids and even numbered chain length fatty acids when cultured in the presence of a carbon source under conditions effective to express the polynucleotides according to (a), (b), and ©, and

wherein at least 10% of the fatty acids in the fatty acid composition are odd-number chain length fatty acids.

Patent Family:
US8372610 US20120070868A1 US20120157717A1 WO2013039563A1 WO2013039563A1